Application

This Anti-LIMS1, clone 5G7 Antibody is validated for use in western blotting, ELISA, flow cytometry & immunofluorescence for the detection of LIMS1.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected LIMS1 in HeLa cells.

Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected LIMS1 in HepG2 cells.

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

LIM and senescent cell antigen-like-containing domain protein 1 (LIMS1), also known as Particularly interesting new Cys-His protein 1 (PINCH-1; PINCH1; PINCH), or Renal carcinoma antigen NY-REN-48 is an adaptor protein which contains five LIM domains, or double zinc fingers. LIMS1 is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that Lims1 may play a role in integrin-mediated cell adhesion or spreading.

Immunogen

Purified recombinant fragment of human LIMS1 expressed in E. Coli

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in A549, Jurkat and HeLa cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected LIMS1 in A549, Jurkat and HeLa cell lysates.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~37 kDa observed. Uncharacterized bands may be observed in some lysate(s).